Immune cells in the lungs provide a rapid counterattack to bloodstream infections, a new study in mice finds. This surprising discovery pegs the lungs as a major pillar in the body’s defense during these dangerous infections, the researchers say.

“No one would have guessed the lung would provide such an immediate and strong host defense system,” says Bryan Yipp, an immunologist at the University of Calgary in Canada. Yipp and his colleagues report their findings online April 28 in Science Immunology.
The work may offer ways to target and adjust our own immune defense system for infections, says Yipp. “Currently, we only try to kill the bacteria, but we are running out of antibiotics because of resistance.”

The research uncovers some of the mechanisms that drive the rapid activation of neutrophils, says immunologist Andrew Gelman of Washington University School of Medicine in St. Louis. “This is critical in removing bacteria from sequestered spaces in the lung,” he says.

Generally, clearing bacteria out of the bloodstream falls to macrophages that reside in the liver and the spleen. But macrophages aren’t found in vessels of the lungs. So the lungs’ blood vessel network gives pathogens a place to hide and escape the body’s usual removal efforts.

In mammals, neutrophils hang out in the lungs’ bloodstream more than in blood vessels that wind through other tissue. Past research has indicated that a dearth of neutrophils puts an individual at risk for a bloodstream infection. It wasn’t clear, though, how they were providing a defensive role in the blood, says Yipp.
Yipp and his team used confocal pulmonary intravital microscopy to view how cells and pathogens behave in living mouse lungs. The researchers found that about a third of the neutrophils seen in the mouse lung sample were crawling about three to four cell lengths along the walls of the capillaries, the smallest blood vessels in the lungs. When the researchers added LPS, a molecule found in the cell wall of gram-negative bacteria like Escherichia coli, almost all of the neutrophils began crawling, and they traveled more than twice as far as before.
One of the proteins needed for neutrophil crawling, CD11b, allows the immune cells to move along as though on a tank tread, says Yipp. After LPS was added, the neutrophils in a mouse lung deficient in CD11b crawled about a third of the distance as the neutrophils in a normal mouse lung.

In another experiment, the researchers injected fluorescent E. coli into a mouse. Within 10 minutes of the bacteria reaching the lungs, the neutrophils started to crawl toward the bacteria and gobble them up, a process called phagocytosis. The neutrophils captured the majority of the bacteria within an hour of injection, says Yipp.

Gelman says it’s now clear that neutrophils are good at removing bacteria from the lungs’ capillaries. The next step is to “show the actual biological impact” — how this system controls a bacterial infection and improves survival, he says.

For patients with depressed immune systems, the finding may eventually provide a way to battle bloodstream infections, Yipp says. But, he notes, “with all infections, part of the concern is that the inflammatory response can become too exuberant, which leads to tissue damage, as in septic shock,” and lung failure. In those cases, learning how to dampen the lungs’ immune response could help, Yipp says.

Seabirds called common murres appear to use preening as a way to negotiate whose turn it is to watch their chick and who must find food. And when one parent is feeling foul, irregularities in this grooming ritual may send the other a signal that all is not well, researchers report in the July issue of The Auk: Ornithological Advances.

“The fascinating part of this study is the inference that communication between mates allows murres to negotiate the level of effort that each member of the pair puts into the breeding effort,” says John Piatt, a wildlife biologist with the U.S. Geological Survey in Anchorage, Alaska. “Reproductive success of this species requires a high degree of cooperation by each mate as they switch duties.”
Common murres (Uria aalge) lay only one egg each breeding season. Parental roles aren’t determined by gender for the birds; mothers and fathers take turns watching over their chick and foraging for fish. When one parent returns with a fish for the chick, the couple preen each other and switch roles. This swapping ceremony typically happens three to four times a day.

But study coauthor Carolyn Walsh noticed that switches don’t always go smoothly. Video of 16 pairs of murres, documenting a total of 198 role swaps, showed that sometimes both birds appeared indecisive. Each parent would hop on and off the chick several times before the birds preened each other and one left to fish. “It’s as if they’re resisting leaving the colony; neither bird actually wants to go,” says Walsh, an animal behavior researcher at Memorial University of Newfoundland in Canada.
For about a fifth of all switching ceremonies, the brooding parent was slow to preen its mate and then refused to switch, forcing the parent that had just returned with a fish to go back out and fish some more.
Irregular behavior also occurred when the parent on fishing duty returned without food, which happened about 10 percent of the time. The empty-beaked bird would quickly start preening its mate, but the mate would be slow to preen back, or might not preen at all. “The brooder is basically communicating, ‘The chick still needs a fish, you better go get one,’” Walsh says.
The ceremony could be a way for the seabirds to communicate their well-being, Walsh says. By withholding preening and delaying the switching ceremony, a murre in poor condition may be trying to negotiate with its partner to have the easier job of brooding. Staying in the nest may allow the bird to rest and recover its strength.

Flying out to sea to fish is energetically costly for murres because they aren’t very aerodynamic. The seabirds are “absolutely ridiculous looking” when they fly, Walsh says. “Their wings are really meant for swimming in the water.”

In physical tests, Walsh and colleagues found a correlation between body condition and ceremony irregularities. Her team captured birds, weighed them and sampled their blood for beta-hydroxybutyrate, a metabolite associated with continual weight loss.

Switching ceremonies lasted about two minutes longer for the lightest birds, around 900 grams, compared with the heaviest birds weighing in at about 1,000 grams. Birds with lower mass and higher metabolite levels also were more likely to preen irregularly, Walsh says.

The longer ceremonies may also be a sign that there’s unrest in the nest. Murres usually mate for life, but pairs can “divorce.” A previous study by Walsh found that mates heading for a split take more time to switch roles.

After a recent flurry of news that fang blennies mix an opioid in their venom, a question lingers: What do they need with fangs anyway? Most eat wimpy stuff that hardly justifies whopper canines.

Not that fang blennies are meek fishes.

“When they bite, they bite savagely,” says Bryan Fry of the University of Queensland in Brisbane, Australia. “If these little jobbies were 3 meters long, we’d be having to cage dive with them.” Real-world blennies, however, grow to only about the size of a cocktail sausage.
These little beasts probably got their big teeth before evolving venom, says Nicholas Casewell of the Liverpool School of Tropical Medicine in England. That’s jusssst backward, snakes might say, as they evolved their venom first. Yet when Casewell, Fry and colleagues put together an evolutionary family tree for the blennies, the one genus with both fangs and venom branched off amid four genera that are all fang and no toxins, Casewell, Fry and colleagues report in the April 24 Current Biology.

Those with venom aren’t that scary to humans. Fish venoms tend to cause excruciating pain, says Fry, who adds from personal experience that “sting” sounds deceptively benign for what a stingray delivers (SN: 4/29/17, p. 28). A venomous fang blenny has yet to nail him, but he hears that others have felt little more than a toothy nip.
“There’s no real reason for most of these fish to have fangs to help them feed,” Casewell says. Many prey on small invertebrates or even floating plankton, which is about as hard to subdue as chicken soup.
The fangs, however, are useful for fending off predators, Casewell suggests. Blennies have no spiky fins or spines, the more usual defensive weapons in fish. Male-versus-male competition may have been another force for fang evolution; males stab each other during breeding season.

When fangs evolved, whatever the reason, they became a useful conduit for venom, Casewell and Fry propose. Once some blennies evolved venom, “all these crazy selection pressures started coming in,” Fry says. Forces of natural selection nudged nonvenomous fang blennies toward colors and stripes similar enough to those of their venomous cousins to discourage attacks from an educated predator.

The mimics take advantage, often brazenly swimming up to bigger fish to bite off some scales and mucus for a snack. “These fish are little jerks,” Fry says. “They should be called jerk blennies.”

The benefits of statins for people older than 75 remain unclear, a new analysis finds. Statins did not reduce heart attacks or coronary heart disease deaths, nor did they reduce deaths from any cause, compared with people not taking statins, researchers report online May 22 in JAMA Internal Medicine.

Recently published guidelines cited insufficient data to recommend statins for people older than age 75 who don’t have a history of cardiovascular disease. The new analysis considered a subset of older adults enrolled in a study of heart attack prevention and mortality conducted from 1994 to 2002. The sample included 2,867 adults ages 65 and older who had hypertension, 1,467 of whom took a statin.

There was no meaningful difference in the frequency of heart attacks or coronary heart disease deaths between those who took statins and those who did not. There was also no significant difference in deaths from any cause, both overall and among participants ages 65 to 74 or those 75 and older.

Statin use may be associated with muscle damage and fatigue, which could especially impact older adults and put them at higher risk for physical decline, the authors say.

For a third time, scientists have detected the infinitesimal reverberations of spacetime: gravitational waves.

Two black holes stirred up the spacetime wiggles, orbiting one another and spiraling inward until they fused into one jumbo black hole with a mass about 49 times that of the sun. Ripples from that union, which took place about 3 billion light-years from Earth, zoomed across the cosmos at the speed of light, eventually reaching the Advanced Laser Interferometer Gravitational-Wave Observatory, LIGO, which detected them on January 4.
“These are the most powerful astronomical events witnessed by human beings,” Michael Landry, head of LIGO’s Hanford, Wash., observatory, said during a news conference May 31 announcing the discovery. As the black holes merged, they converted about two suns’ worth of mass into energy, radiated as gravitational waves.
LIGO’s two detectors, located in Hanford and Livingston, La., each consist of a pair of 4-kilometer-long arms. They act as outrageously oversized rulers to measure the stretching of spacetime caused by gravitational waves. According to Einstein’s theory of gravity, the general theory of relativity, massive objects bend the fabric of space and create ripples when they accelerate — for example, when two objects orbit one another. Gravitational ripples are tiny: LIGO is tuned to detect waves that stretch and squeeze the arms by a thousandth of the diameter of a proton. Black hole collisions are one of the few events in the universe that are catastrophic enough to produce spacetime gyrations big enough to detect.
The two black holes that spawned the latest waves were particularly hefty, with masses about 31 and 19 times that of the sun, scientists report June 1 in Physical Review Letters. LIGO’s first detection, announced in February 2016, came from an even bigger duo: 36 and 29 times the mass of the sun (SN: 3/5/16, p. 6). Astrophysicists don’t fully understand how such big black holes could have formed. But now, “it seems that these are not so uncommon, so clearly there’s a way to produce these massive black holes,” says physicist Clifford Will of the University of Florida in Gainesville. LIGO’s second detection featured two smaller black holes, 14 and eight times the mass of the sun (SN: 7/9/16, p. 8).
Weighty black holes are difficult to explain, because the stars that collapsed to form them must have been even more massive. Typically, stellar winds steadily blow away mass as a star ages, leading to a smaller black hole. But under certain conditions, those winds might be weak — for example, if the stars contain few elements heavier than helium or have intense magnetic fields (SN Online: 12/12/16). The large masses of LIGO’s black holes suggest that they formed in such environments.

Scientists also disagree about how black holes partner up. One theory is that two neighboring stars each explode and produce two black holes, which then spiral inward. Another is that black holes find one another within a dense cluster of stars, as massive black holes sink to the center of the clump (SN Online: 6/19/16).

The new detection provides some support for the star cluster theory: The pattern of gravitational waves LIGO observed hints that one of the black holes might be spinning in the opposite direction from its orbit. Like a cosmic do-si-do, each black hole in a pair twirls on its own axis as it spirals inward. Black holes that pair up as stars are likely to have their spins aligned with their orbits. But if the black holes instead find one another in the chaos of a star cluster, they could spin any which way. The potentially misaligned black hole LIGO observed somewhat favors the star cluster scenario. The measurement is “suggestive, but it’s not definite,” says astrophysicist Avi Loeb of Harvard University.

Scientists will need more data to sort out how the black hole duos form, says physicist Emanuele Berti of the University of Mississippi in Oxford. “Probably the truth is somewhere in between.” Various processes could contribute to the formation of black hole pairs, Berti says.

As with previous detections of gravitational waves, the scientists used their measurements to test general relativity. For example, while general relativity predicts that gravitational waves travel at the speed of light, some alternative theories of gravity predict that gravitational waves of different energies travel at different speeds. LIGO scientists found no evidence of such an effect, vindicating Einstein once again.

Now, with three black hole mergers under their belts, scientists are looking forward to a future in which gravitational wave detections become routine. The more gravitational waves scientists detect, the better they can test their theories. “There are already surprises that make people stop and revisit some old ideas,” Will says. “To me that’s very exciting.”

Paper wasps have a knack for recognizing faces, and a new study adds to our understanding of what that means in a wasp’s brain.

Most wasps of a given species look the same, but some species of paper wasp (Polistes sp.) display varied colors and markings. Recognizing these patterns is at the core of the wasps’ social interactions.

One species, Polistes fuscatus, is especially good at detecting differences in faces — even better than they are at detecting other patterns. To zero on the roots of this ability, biologist Ali Berens of Georgia Tech and her colleagues set up recognition exercises of faces and basic patterns for P. fuscatus wasps and P. metricus wasps — a species that doesn’t naturally recognize faces but can be trained to do so in the lab. After the training, scientists extracted DNA from the wasps’ brains and looked at which bits of DNA or genes were active.

The researchers found 237 genes that were at play only in P. fuscatus during facial recognition tests. A few of the genes have been linked to honeybee visual learning, and some correspond to brain signaling with the neurotransmitters serotonin and tachykinin. In the brain, picking up on faces goes beyond basic pattern learning, the researchers conclude June 14 in the Journal of Experimental Biology.

It’s possible that some of the same genes also play a broader role in how organisms such as humans and sheep tell one face from another.

Ancient rock artists were drawn to echo chambers. Members of early farming communities in Europe painted images in rock-shelters where sounds bounced off walls and into the surrounding countryside, researchers say.

Rock-shelters lacking such sound effects were passed up, at least in the central Mediterranean, report archaeologist Margarita Díaz-Andreu of the University of Barcelona and colleagues in the July Journal of Archaeological Science. In landscapes with many potential rock art sites, “the few shelters chosen to be painted were those that have special acoustic properties,” Díaz-Andreu says.
Some hunter-gatherer and farming groups studied over the past couple centuries believed in spirits that dwell in rock and reveal their presence via echoes. But acoustic evidence of special echoing properties at rock art sites is rare.

Díaz-Andreu’s team studied two rock art sites in 2015 and 2016. Baume Brune is a kilometer-long cliff in southeastern France. Of 43 naturally formed cavities in the cliff, only eight contain paintings, which include treelike figures and horned animals. Rock art in the Valle d’Ividoro, on Italy’s east coast, appears in an 800-meter-long section of a gorge. Only three of its 11 natural shelters contain painted images. Researchers generally date these French and Italian paintings to between roughly 6,500 and 5,000 years ago, several thousand years after the Stone Age had ended, Díaz-Andreu says.

To investigate the acoustics of the decorated and unadorned shelters, the researchers developed a new technique for determining the direction, intensity and timing of sound waves arriving at a particular point from every direction. A special microphone connected to a digital recorder measured the acoustic properties of any echoes set off by balloons popped just outside each rock-shelter. This setup was moved to various spots outside the caves to record the acoustic reach of reflected popping sounds. Echo measurements in France were taken at distances ranging from 22 to 36 meters from cliff shelters. Due to rougher terrain in Italy, measurements there were taken at distances ranging from 77 to 300 meters.
Then, the acoustic data were transformed into 3-D, slow-motion depictions of echoes, represented by moving circles, indicating where sound reflections originated. At both sites, shelters with rock paintings displayed better echoing properties than undecorated shelters, Díaz-Andreu says. And in each location, the shelter that best reflected echoes had the highest number of paintings.
“This novel technique shows a clear correlation between audible echoes and decorated shelters,” says music archaeologist Riitta Rainio of the University of Helsinki in Finland, who did not participate in the new study.

Echoes that bounce off steep rock cliffs bordering three lakes in northern Finland also attracted ancient artists, Rainio says. She and her colleagues took acoustic measurements at Finland’s painted cliffs from 2013 to 2016. Microphones placed on boats positioned at different spots on nearby lakes measured sound waves generated, in most trials, by a starter’s pistol. These Finnish paintings date to between around 7,200 and 3,000 years ago, Rainio says.

In some cases, echoes reflect directly from cliff paintings. “That, and possible drumming figures painted on the cliffs, suggest that sound played some role in rituals at these sites,” Rainio says. Her team will report its findings in an upcoming Journal of Archaeological Method and Theory.

Creators of older Stone Age cave art also appear to have focused on sites where echoes abounded, says archaeologist Paul Pettitt of Durham University in England. For instance, many roughly 14,000- to 12,000-year-old animal drawings and engravings at France’s Niaux Cave cluster in a cathedral-like chamber where sounds echo loudly.

“The new study provides convincing evidence that echoes, which were scientifically inexplicable to prehistoric people, played a determining role in how art was created,” Pettitt says.

Babies are little heartbreakers — literally. Genetic variants linked to fertility are also linked to coronary artery disease, a new study finds.

It’s not uncommon to find genes that affect more than one trait, but this is the first time scientists have seen a genetic connection between reproduction and heart disease, the researchers report online June 22 in PLOS Genetics. “Evolution is on a buy now, pay later plan,” says coauthor Stephen Stearns, an evolutionary biologist at Yale University. The connection “leads to a view of us as a bundle of trade-offs,” he says. And in this case, genes’ reproductive benefits apparently outweigh even lethal side effects later in life.
Coronary artery disease — one of the most deadly diseases worldwide — results from plaque accumulation in the arteries that supply blood to the heart. That type of buildup, which can start in young adulthood, has afflicted humans for millennia, and scientists have wondered why the genetic variants complicit in the disease haven’t yet been weeded out of the gene pool.

“There must be some advantage to these genes that makes them worth keeping,” says Shari Grossman, a geneticist at the Broad Institute in Cambridge, Mass., who was not involved in the study.

Researchers examined genetic variants associated with coronary artery disease and found evidence that they spread rapidly through the human population within the last 10,000 years or so. This implies that sometime in relatively recent human history these tweaked genes provided an evolutionary advantage.

Then the researchers reviewed 143 previous studies and discovered many of these same genes were linked to — and probably enhanced — important reproductive functions, such as male and female fertility as well as fetal development and survival. This suggests that the genetic quirks associated with coronary artery disease persisted because the people who had them bore more children.

Having birthrate-boosting genes was probably particularly advantageous in the last several thousand years because of the transition to agriculture, Stearns says. Agriculture led to people settling down and eventually moving to cities, where rampant infectious diseases hiked mortality rates, especially among children. People with these genetic variants would have been more likely to continue their lineage, even if they were predisposed to suffer heart problems later on.
This study may be a warning for gene therapy, since it suggests there are many genetic connections between different bodily functions that scientists don’t yet understand, Stearns says. If doctors want to treat coronary artery disease by editing a person’s DNA, it’s important to know what other traits might be affected.

The new findings also raise questions about the various functions of other disease-related genes, says Hamdi Mbarek, a molecular geneticist at Vrije University Amsterdam who was not involved in the work. For instance, a future study could examine whether genes associated with cancer have any hidden evolutionary benefits.

A bad bacterium may make colon cancer worse.

Streptococcus gallolyticus spurred growth of some colon cancer cells in lab dishes and in mice, researchers report July 13 in PLOS Pathogens. S. gallolyticus stimulates a biochemical chain reaction that scientists already knew is involved in the development of colon cancer, the researchers discovered.

Bacteria had to be in direct contact with tumor cells to speed growth, but exactly how the bacteria do that isn’t yet known. Further investigation could help researchers find ways to block the microbe’s action, leading to better treatments for colorectal cancer, says microbiologist Yi Xu of Texas A&M University Health Science Center in Houston.
People who have heart valve or blood infections of S. gallolyticus (previously known as S. bovis) often also have colorectal tumors. The bacterium has also been found growing on tumors in some colorectal cancer patients. But doctors couldn’t tell from association studies whether the bacteria were egging on tumors or were innocent bystanders.

Xu and colleagues grew S. gallolyticus in lab dishes with several different types of human cells. Three types of colon cancer cells grew faster, producing about 50 to 60 percent more cells within 24 hours, with the bacteria than they did when cultured with no bacteria or with a harmless, milk-fermenting bacterium called Lactococcus lactis. Normal human colon cells, kidney cells, lung cancer cells and two strains of colon cancer cells didn’t respond to the bacteria. Those results could mean that the bacterium doesn’t spur on all colon cancers, says Cynthia Sears, an infectious disease specialist at Johns Hopkins University School of Medicine who was not involved in the work. Finding out what makes some cells more vulnerable to the bacteria will be important for future studies, she says.

“It deserves a deeper look,” Sears says.

Colon cancer‒prone mice infected with S. gallolyticus had more and bigger tumors than those found in mice inoculated with L. lactis or with a saline solution.

Xu and colleagues don’t know all the details of how S. gallolyticus promotes colon cancer growth. But the researchers discovered that when the bacteria glom onto responsive colon cancer cells, the microbes boost a signal sent through a relay chain known as the beta-catenin pathway. That pathway was already known to be involved in generating colorectal tumors. The researchers have some evidence that S. gallolyticus may also work through other chemical signaling pathways to enhance colon cancer growth.
Whether the bacterium can initiate colon cancer isn’t clear, Xu says.

Researchers will also need to investigate how S. gallolyticus works with or against other microbes that live in the colon, says Ran Blekhman, a geneticist at the University of Minnesota in Minneapolis. The study is part of a growing trend away from merely associational studies toward discovering how microbes function in the body, he says. “This is basically the next step in microbiome research.”

Big, rogue planets — ones without parent stars — are rare.

A new census of free-floating Jupiter-mass planets determined that these worlds are a tenth as common as previous estimates suggested. The results appear online July 24 in Nature.

Planets can go rogue in two ways: They can get kicked out of their parent planetary systems or form when a ball of gas and dust collapses (SN: 4/4/15, p. 22).

In the new study, Przemek Mróz of the Astronomical Observatory of the University of Warsaw and colleagues estimated the number of large, rogue planets in our galaxy using a technique called microlensing. When an object with a mass of a planet passes in front of a distant, background star, the gravity of the planet acts as a gravitational magnifying glass. It distorts and focuses the light, giving up the planet’s existence.
Mróz and colleagues looked at 2,617 microlensing events recorded between 2010 and 2015 and determined which were caused by a rogue planet. For every typical star, called main sequence stars, there are 0.25 free-floating Jupiter-mass planets, the analysis suggests.

The new result sharply contrasts an estimate published in 2011, which suggested that rogue Jupiters are almost twice as common as main sequence stars. About 90 percent of stars in the universe are main sequence stars, so if that estimate were accurate, there should be a lot of free-floating Jupiters.

“That result changed our conceptual framework of the universe just a little bit,” says astronomer Michael Liu of the University of Hawaii in Honolulu. It challenged long-held ideas about how planets go rogue because the known methods wouldn’t generate enough planets to account for all the wanderers.

The 2011 result was based on a relatively small sample of microlensing events, only 474. Since then, infrared telescope images haven’t detected as many free-floating planets as expected. “Over the years, serious doubts were cast over the claims of a large population of Jupiter-mass free-floaters,” Mróz says.

David Bennett, coauthor of the 2011 study, agrees that the new census failed to find evidence for a large population of Jupiter-mass rogue planets. He notes, however, that the new data do reveal four times as many Jupiter-mass failed stars called brown dwarfs than predicted in the original census. So some of the rogues that were originally classified as planets may, in fact, be failed stars. Bennett, of NASA’s Goddard Space Flight Center in Greenbelt, Md., and colleagues are working on a new analysis of potential rogues with nearly 3,000 microlensing events and plan to compare their results with those from the new census.
Liu says the latest census is much more in line with theories of how planets form. Most rogues should be Earth-mass or a little heavier. Those lighter planets get tossed out of their planetary systems much easier than behemoths like Jupiter. Still, the smaller planets are harder to detect.

The new microlensing analysis did identify several events in which stars brightened and dimmed in less than half a day. Such short events hint at the existence of Earth-mass free-floaters because smaller planets with less gravity should brighten a distant star more briefly than more massive stars. Determining whether those small planets are really rogue and counting how many there are will take better telescopes, the team notes.